Partillion Bioscience: a new single-cell technology
Nanovials; Secretome; scRNAseq
Introduction
One of the main goals in biomedical research is to understand how cells work, both individually and within tissues or organisms. This includes not only how cells function but also how they 🗣️ with their neighbours and surroundings. Cells do this by releasing proteins and molecules, known as the secretome. The secretome is 🔑 for communication, shaping the environment, and defending cells against threats.
Studying the secretome at the single cell level has been difficult due to technical challenges. But with new technology from Partillion Bioscience, we can now study it on a large scale (10,000 to 1,000,000 cells per experiment). Plus, we can combine it with other technology like single-cell sequencing. Ready to know how?
Technology
Nanovials
A nanovial is a tiny transparent hydrogel ball with a cavity designed to hold a single cell. In addition to cells, the cavities can hold small amounts of liquid (<1 nl), allowing them to function like mini-test tubes for various reactions.
There are 2 types of nanovials (Figure 1). The original type has low autofluorescence and cavities with hundreds of millions of biotinylated binding sites. The EZM type is recommended for adherent cells and, in addition to biotinylated binding sites, has an extracellular matrix coating.
Partillion's nanovials come in two cavity sizes: 18 μm and 28 μm (Figure 2). The larger cavities can fit two cells, enabling multi-cell assays.
