scGET-seq: Studying Chromatin at Higher Resolution

In this podcast episode, I talk with Francesca Giannese about an exciting new method for studying chromatin at the single-cell level called scGET-seq. Unlike previous methods such as scATAC-seq, which only allow us to study open chromatin, scGET-seq provides insights into both open and closed chromatin. This breakthrough can lead to a deeper understanding of how chromatin structure impacts gene regulation in individual cells. Tune in to learn more about how scGET-seq works and its potential applications in research.

Contacts for support on scGET-seq implementation:

• Francesca Giannese (wet-lab protocol implementation - enzyme requests)

• Davide Cittaro (data analysis)

More information about scGET-seq

• scGET-seq Wikipedia

• Original Paper

Data Analysis

• Analyzing genomic and epigenetic profiles in single cells by hybrid transposase (scGET-seq)

• Analysis and modeling of scGET-seq data 

Application

• Cancer evolution (EMT in pancreatic cancer)